Nyob Cell Intelligent Scanning System

Lub cev muaj sia nyob ntawm tes tuaj yeem muab tso rau hauv CO₂ incubator los tsim ib qho chaw sim hauv lub incubator. Nws yog siv rau microscopic scanning thiab kev soj ntsuam ntawm lub sijhawm tiag tiag ntawm cov txheej txheem culturing ntawm cov cell nyob thiab rau qhov kom muaj nuj nqis ntawm cov hlwb. Thaum lub sij hawm tag nrho culturing lub sij hawm, real-time cov ntaub ntawv hais txog kev loj hlob ntawm tes yuav tau remotely. Cov kev tshawb fawb kev txawj ntse nyob rau hauv lub cell imaging system yuav siv tau los xam cov xov tooj ntawm tes thiab confluence. Piv nrog rau cov txheej txheem kab lis kev cai ntawm tes, nws tuaj yeem txuag tau ntau tus nqi ntawm ob lub sijhawm thiab nyiaj txiag. Nws ua rau cov kws tshawb fawb kom nkag siab txog kev noj qab haus huv, morphology thiab kev ua haujlwm ntawm cov qauv ntawm tes tau zoo dua thiab muaj zog.

1. Nyob - cell imaging function

Hom kev yees duab

Lub Live Cell Intelligent Scanning system txhawb ntau hom duab. Ntawm lawv, fluorescence imaging muaj peev xwm tshwj xeeb sau ntau yam biomolecules nyob rau hauv lub cell, ua tiav siab - rhiab heev thiab siab - daws teeb meem visualization. Theem - kev sib piv cov duab ua rau kev soj ntsuam ntawm cov morphology thiab cov qauv ntawm cov hlwb nyob tsis tas yuav tsum tau staining.

2.Kev tsom xam muaj nuj nqi

Tsis siv neeg lub xov tooj lees paub thiab taug qab Lub Live Cell Intelligent Scanning System yog nruab nrog cov duab paub txog cov algorithms. Nws tuaj yeem txheeb xyuas ntau hom ntawm cov cell nyob thiab nrhiav tau lawv qhov tseeb txawm tias nyob hauv ib puag ncig ntawm cov cellular complex. Los ntawm kev taug qab mus tas li, cov dynamic trajectories ntawm cov hlwb nyob rau hauv ob peb teev lossis txawm hnub tuaj yeem raug kaw tag nrho.

3.Quantitative tsom xam muaj nuj nqi

Lub Live Cell Intelligent Scanning System tuaj yeem ua qhov kev soj ntsuam kom muaj nuj nqis ntawm ntau lub hlwb. Cov kws tshawb fawb tuaj yeem yooj yim tau txais cov ntaub ntawv txheeb xyuas ntawm cov pej xeem ntawm tes los ntawm nws, suav nrog kev hloov pauv ntawm cov xov tooj ntawm tes, qhov sib txawv ntawm cov xov tooj ntawm tes thiab cov duab nyob rau hauv cov kev kho mob sib txawv, thiab cov ntaub ntawv ntau ntawm cov lus qhia theem ntawm fluorescent - cov ntawv lo molecules.

Optical Cheebtsam
1.Microscope lub cev
Lub Live Cell Intelligent Scanning System tau txais kev tsim kho lub tshuab ntsuas qhov siab tshaj plaws thiab tau nruab nrog lub tshuab kho qhov muag infinity, ua kom muaj kev ruaj ntseg ntawm txoj kev teeb pom kev zoo thiab cov duab zoo. Nws tseem tau nruab nrog tshwj xeeb-ua high-tus lej-aperture lo ntsiab muag thiab cov ntxaij lim dej zoo, txhim kho lub teeb-siv efficiency thiab qhov tshwj xeeb ntawm cov duab.
 

2. Lub teeb qhov system
Lub Live Cell Intelligent Scanning System yog nruab nrog lub teeb ci siab, lub neej ntev LED qhov chaw nrog kho lub teeb siv thiab ntau txoj kev xaiv wavelength. Nws tuaj yeem tsis tsuas yog ua tau raws li qhov yuav tsum tau ua ntawm cov qauv duab sib txawv tab sis kuj txo qis kev puas tsuaj rau lub hlwb.
 

3. Automated Cheebtsam
1.Stage Lub siab - precision motorized theem tuaj yeem ua tiav qhov kev txav mus los hauv X thiab Y cov lus qhia, thiab tuaj yeem ua kom pom tseeb hauv Z - axis kev taw qhia, ua tau raws li kev xav tau ntawm cov hlwb ntawm ntau qib. Nyob rau tib lub sijhawm, nws txhawb nqa txoj haujlwm preset thiab tsis siv neeg scanning muaj nuj nqi, ua kom yooj yim rau kev soj ntsuam ntawm cov qauv loj.

2. Auto - focusing system Lub pib - focusing system yog ua raws li cov duab tsom xam algorithms siab heev, uas tuaj yeem ceev thiab raug kaw lub xovtooj ntawm tes kom pom tseeb thiab tswj xyuas cov duab meej meej thaum lub sijhawm soj ntsuam ntev yam tsis tas yuav tsum tau hloov kho ntau zaus.

4. Kab lis kev cai
Cell kab lis kev cai Chamber Lub cell kab lis kev cai chamber yog ua los ntawm high-zoo biocompatible ntaub ntawv, nrog zoo huab cua permeability thiab tshav kub hloov zog. Cov qauv tsim tshwj xeeb tuaj yeem ua raws li kab lis kev cai ntawm ntau yam kev qhia tshwj xeeb, tso cai rau cov neeg siv xaiv raws li kev sim xav tau.

1. Kev ua haujlwm interface
Cov neeg siv-phooj ywg
Lub Live Cell Intelligent Scanning System muaj qhov ua haujlwm yooj yim thiab nkag siab zoo, ua rau cov kws tshawb fawb tsis muaj kev paub txog kev paub ntau yam kom pib sai. Cov ntawv qhia meej meej thiab lub cim tsim ntawm Live Cell Intelligent Scanning System pab cov neeg siv hauv kev teeb tsa ntau yam, xaiv hom duab, thiab ua haujlwm khaws cov ntaub ntawv.

2.Function modules
Cov duab tswj module
Lub imaging tswj module tso cai rau cov neeg siv los kho kom zoo ntawm lub sijhawm raug thiab yooj yim teeb tsa cov duab sib luag, nrog rau lub sijhawm luv luv, hloov pauv hom duab, thiab lwm yam, kom tau raws li kev sim ntau yam.

3.Kev tsom xam module
Lub tsom xam module yog seamlessly integrated nrog cov ntaub ntawv imaging. Cov neeg siv tuaj yeem tshawb xyuas tam sim tom qab sau cov ntaub ntawv. Los ntawm kev ua haujlwm yooj yim, cov ntaub ntawv txheeb xyuas cov txiaj ntsig tau zoo, xws li cov duab kos duab ntawm tes thiab cov fluorescence siv cov histograms, tuaj yeem tsim tau, pab cov kws tshawb fawb kom tau txais cov ntaub ntawv tseem ceeb sai.

1. Cell biology tshawb fawb
Kev tshawb fawb txog cellular physiological txheej txheem
Lub Live Cell Intelligent Scanning System tau siv dav hauv kev tshawb fawb ntawm tes biology. Piv txwv li, hauv kev tshawb fawb txog kev loj hlob ntawm tes, nws tuaj yeem tshawb xyuas lub sijhawm tiag tiag ntawm txhua theem ntawm kev faib cell thiab suav lub sijhawm ntawm lub voj voog ntawm tes. Hauv kev tshawb fawb ntawm tes apoptosis, los ntawm fluorescently sau npe apoptosis-txog cov proteins, nws tuaj yeem ntes cov kev hloov morphological thiab molecular mechanisms ntawm cell apoptosis.

2.Drug research and development
Kev tshuaj ntsuam yeeb tshuaj thiab tshuaj ntsuam xyuas kev ua tau zoo
Hauv kev tshawb nrhiav tshuaj thiab kev tsim kho, lub kaw lus tuaj yeem siv rau kev tshuaj ntsuam xyuas tshuaj ntau dhau. Los ntawm kev soj ntsuam cov kev hloov pauv hauv cell morphology thiab kev muaj peev xwm ua tau zoo tom qab kev kho tshuaj thiab kev sib cuam tshuam ntawm cov tshuaj thiab cov hom phiaj, qhov ua tau zoo ntawm cov tshuaj tuaj yeem raug soj ntsuam sai, muab kev txhawb zog rau kev tshawb fawb tshuaj thiab kev loj hlob.

3. Kev tshawb fawb txog microbiology
Kev soj ntsuam ntawm kev loj hlob thiab kev coj tus cwj pwm ntawm cov kab mob
Rau kev tshawb fawb microbiology, nws tuaj yeem tshawb xyuas qhov kev loj hlob ntawm cov kab mob, cov txheej txheem biofilm tsim, thiab kev loj hlob ntawm hyphal thiab spores germination ntawm fungi, pab kom nkag siab tob tob txog kev ua neej txoj cai ntawm cov kab mob.

1. Kev teeb tsa thiab kev cob qhia

• Kev pab cuam installation

Peb muab cov kws tshaj lij kev teeb tsa pab pawg, uas yog lub luag haujlwm rau kev txhim kho Live Cell Intelligent Scanning System ntawm cov neeg siv khoom tshwj xeeb qhov chaw thiab ua cov kev debugging kom paub meej tias lub kaw lus ua haujlwm zoo tshaj plaws.

• Kev pabcuam kev cob qhia

Peb muab cov neeg siv nrog cov kev pabcuam kev cob qhia, suav nrog kev qhia ua haujlwm hauv online thiab offline ntawm - qhov chaw kawm. Cov ntsiab lus kev cob qhia suav nrog kev ua haujlwm yooj yim ntawm lub cev, kev siv cov haujlwm siab heev, thiab cov txheej txheem kev tshuaj xyuas cov ntaub ntawv. Lub sijhawm kev cob qhia tuaj yeem hloov kho raws li cov neeg siv yuav tsum tau ua kom ntseeg tau tias cov neeg siv tuaj yeem txawj siv lub system.

2.After - kev muag khoom kho thiab tu

• Kho kev pabcuam

Peb muaj ib pab neeg ua haujlwm kho tom qab muag rau Live Cell Intelligent Scanning System, uas yuav teb tsis pub dhau 2 teev tom qab tau txais daim ntawv thov kho txog lub kaw lus. Peb muaj cov khoom tsim kho lub Hoobkas txaus txaus hauv Tshuag rau Live Cell Intelligent Scanning System, uas tuaj yeem daws tau qhov system tsis ua haujlwm ntawm nws sai thiab txo qis sijhawm.

• Kev pab tu

Peb muab cov kev pabcuam saib xyuas tsis tu ncua thiab tsim cov phiaj xwm kev saib xyuas tus kheej raws li kev siv ntawm lub kaw lus, suav nrog kev tu cov khoom siv kho qhov muag, lubrication thiab calibration ntawm cov khoom siv tshuab, kom ntseeg tau tias lub sijhawm ua haujlwm ruaj khov ntawm lub cev.

Citation ntawm kev ntsuam xyuas cov neeg siv khoom
Cov neeg siv khoom tawm tswv yim qhia tias peb lub kaw lus yooj yim rau kev khiav lag luam, muaj cov duab zoo tshaj plaws, thiab pab pawg txhawb nqa kev teb sai, txhim kho lawv cov kev tshawb fawb zoo heev.

 

Stable theem
Tau txais cov duab meej dua nrog lub phaj ruaj khov. Tsis zoo li lwm yam khoom siv, cov duab nyob ntawm tes muaj lub sijhawm ruaj khov thiab cov optics txav mus.
Siab compatibility
Tau tshaj ntau hom kab lis kev cai ntawm tes. Cov phaj zoo, tais, thiab T-flask tuaj yeem xaiv tau.
Cwj pwm thiab ua haujlwm hauv lub sijhawm
Live-cell imaging ua rau cov kws tshawb fawb los kawm txog cov txheej txheem ntawm tes, kev coj cwj pwm thiab kev ua haujlwm ntawm lub sijhawm thiab sijhawm dhau sijhawm, yog li muab qhov pom tseeb ntawm kev ua haujlwm lom neeg.
Muaj peev xwm txheeb xyuas txhua lub sijhawm
Kinetic nyob-cell imaging zam qhov yuav tsum tau npaj ib qho qauv cais rau txhua lub sij hawm taw tes los soj ntsuam - ib qho qauv tuaj yeem txheeb xyuas lub sijhawm..

Lub optics system muaj kev mus ncig ntawm 117mm raws li x-axis thiab 77mm raws y-axis. Ntau cov ntsiab lus nyob rau hauv qhov ntau no tuaj yeem ntes tau raws li lub sijhawm (nrog rau lub sijhawm, lub voj voog, thiab tag nrho lub sijhawm) txiav txim los ntawm tus kws tshawb fawb. Ntau yam ntawm cov hlab ntsha xws li cov phaj zoo, tais diav, flasks, thiab slides tuaj yeem siv. Nyob rau hauv lub cell imaging system, es tsis txhob ntawm ib tug movable theem, lub koob yees duab nyob rau hauv lub system tsiv mus rau tau txais cov duab ntawm lub hlwb nyob rau hauv ntau txoj hauj lwm. Nrog rau kev sib koom ua ke nyuaj - coated optical Cheebtsam thiab LED lim uas muaj lub neej ntev tshaj 50, 000 teev, meej thiab rhiab fluorescence nrhiav tau ua tiav. Lub cev duab nyob ntawm tes yog qhov loj me, nrog qhov ntev ntawm 226mm hauv qhov siab, 358mm hauv ntev, thiab 215mm dav. Ntau lub tshuab AutoLCI tuaj yeem muab tso rau hauv tus qauv CO2 incubator. Kev tswj xyuas qhov ua tau zoo ntawm cov cuab yeej hauv qhov chaw kub thiab av noo yog qhov nyuaj heev. Txawm li cas los xij, nrog AutoLCI, koj tuaj yeem siv zog saib xyuas cov cell nyob hauv lub incubator rau lub sijhawm txuas ntxiv yam tsis muaj kev cuam tshuam nrog ib puag ncig zoo rau kev coj noj coj ua ntawm tes. Daim ntawv thov scanning yog ua haujlwm rau kev ntes duab. Los ntawm ib qho screen intuitive nkaus xwb, koj tuaj yeem saib ua ntej cov hlwb, npaj cov duab - ntes cov sijhawm, kho lub teeb thiab sib piv, thiab ua raws sijhawm - lapse kev nce qib. Nws suav nrog nws pib - tsom cov thev naus laus zis uas tuaj yeem txheeb xyuas lub dav hlau pom tseeb ntawm cov hlwb thiab muaj qhov rov ua tau zoo kawg.

Peb nthuav qhia txoj hauv kev tshiab thiab thev naus laus zis los pab koj kom ncav cuag koj lub hom phiaj kev tshawb fawb thiab kev txhim kho. Peb cov khoom siv hluav taws xob siv hluav taws xob zoo tshaj plaws muab cov duab zoo tshaj plaws ntawm txhua lub xov tooj ntawm tes muaj nyob hauv khw. Thaum ua ke nrog lub xeev-of-the-art software pob khoom thiab cov kev daws teeb meem automation, lawv tuaj yeem lav qhov kev txhawb nqa zoo tshaj plaws hauv koj cheeb tsam tshwj xeeb. Hauv thaj tsam ntawm txoj kev loj hlob ntawm tes, piv txwv suav nrog tib lub xov tooj ntawm tes cloning, kev txheeb xyuas ntawm monoclonality, taug qab ntawm CRISPR / Cas9, kev ntsuam xyuas ntawm kev hloov pauv ntawm tes, kev saib xyuas ntawm cell viability, ntsuas PAIA protein titer, ntsuas ntawm PAIA glycosylation, thiab fluorescent activated ib leeg. cloning (FASCC). Rau kev tshawb nrhiav mob qog noj ntshav thiab tshawb nrhiav tshuaj, cov dej num xws li kev tshuaj xyuas 3D spheroids, kuaj tshuaj lom neeg, IC50 kev tshawb fawb, taug qab kev nthuav dav ntawm tes, saib xyuas apoptosis, tus cwj pwm ntawm cov nucleus, kev kho qhov txhab thiab kev tsiv teb tsaws, kawm H2AX-DNA kev puas tsuaj, thiab tshuaj xyuas. Lub voj voog ntawm tes thiab mitosis koom nrog. Hauv kev tshawb fawb qia cell, kev ua ub no xws li suav iPS cov cheeb tsam, ua cov kev tshawb fawb fluorescent pluripotency, validating proliferation thiab cell migration, ntsuam xyuas cell txawv, siv recombinant lectin probes, suav cornea hlwb, kuaj siRNA, thiab characterizing iPS-cell markers yog nqa tawm. Hauv kev tiv thaiv kab mob, kev tshawb fawb ntawm B-cells thiab T-cells, kev sim ntawm cytotoxic T-lymphocytes, kev ntsuam xyuas ntawm tus pab T-cells thiab lawv cov subsets, thiab ua cov kev tshawb fawb ntawm cell tuag. Hauv kev tshawb fawb tshuaj tiv thaiv, kev ua haujlwm xws li siv qhov kev ntsuam xyuas ua kom pom tseeb (FFA) txhawm rau txheeb xyuas tus kab mob titer, ua haujlwm ntawm immunofluorescence foci assay (IFA) txhawm rau txheeb xyuas tus kab mob kis, ua cov tshuaj tiv thaiv kab mob, tshawb xyuas kab mob kis los ntawm kev txiav txim siab txog kev hloov pauv hloov pauv, nrog fluorescence-coupled gene qhia, thiab ntsuas cov nyhuv cytopathic (viral CPE) tau ua.

Thaum lub sij hawm nyob hauv cell imaging, cov hlwb nyob tau raug soj ntsuam rau ib lub sij hawm nyob rau hauv ib tug nyob cell imaging microscope. Txhawm rau ua kom muaj kev ua haujlwm tsis siv neeg hauv cov duab nyob ntawm tes, cov kev daws teeb meem nyob hauv cell tam sim no feem ntau suav nrog kev tshawb fawb lub tshuab hluav taws xob, uas suav nrog lub koob yees duab microscope, nrog rau cov software tshwj xeeb rau kev tsim thiab ua qhov kev sim thiab tshuaj xyuas cov ntaub ntawv. Nyob rau ib lub sij hawm ntev, cov duab ntawm ib qho chaw saib lossis tag nrho cov qauv hauv cheeb tsam raug kaw ib qho tom qab lwm lub sijhawm tshwj xeeb. Txhawm rau tswj cov hlwb hauv lub cev lub cev thoob plaws qhov kev sim, cov kab ke ntawm cov duab nyob hauv feem ntau tuaj nrog cov chaw tsim hluav taws xob uas tuaj yeem tswj qhov kub, av noo, thiab CO₂ concentration. Nws yog ib qho tseem ceeb uas cov kev txwv no tuaj yeem hloov kho raws li cov kev xav tau ntawm cov hlwb thiab khaws cia nyob rau theem tsis tu ncua thaum lub sijhawm sim tag nrho.

Cells tuaj yeem ua duab siv ntau hom duab. Piv txwv li, brightfield microscopy tuaj yeem txhawb nqa los ntawm txoj hauv kev zoo li theem sib piv. Tsis tas li ntawd, ob peb lub xov tooj ntawm tes kev yees duab tau tsim nrog kev siv cov tshuaj fluorescent tshwj xeeb rau cov duab nyob ntawm tes. Cov tswv yim no tuaj yeem txheeb xyuas cov hlwb uas txaus siab thiab xaiv los saib xyuas kev txhim kho, sib txawv, lossis kev muaj peev xwm ntawm cov hlwb. Yog li ntawd, nyob hauv cell fluorescence microscopy yog ib qho cuab yeej siv tau zoo uas tuaj yeem nthuav tawm ntau cov ntaub ntawv ntxiv txog tib neeg lub hlwb. Live cell super - daws teeb meem microscopy los yog 3D nyob ntawm tes imaging tuaj yeem muab kev nkag siab ntau dua thiab cov kev xav tshiab rau kev tsom xam ntawm cov cell nyob.

Cov duab kaw tuaj yeem nkag mus, saib, thiab tshuaj xyuas nrog cov khoom siv ntawm tes tsom xam software. Ib cov duab ntawm ib leeg tuaj yeem hloov mus rau hauv cov yeeb yaj kiab hauv cell imaging, thiab software algorithms tuaj yeem ua cov ncauj lus kom ntxaws ntawm cov hlwb nyob rau lub sijhawm, xws li cov kab mob ntawm cov cell tsiv teb tsaws. Yog li ntawd, nyob rau hauv lub cell imaging, lub sij hawm tsis yog ib tug ntxiv dimension; Nws ua rau peb soj ntsuam cov txheej txheem uas yuav tsis tuaj yeem tshawb pom.

Peb nthuav qhia cov tswv yim zoo thiab thev naus laus zis los pab koj kom ua tiav koj lub hom phiaj kev tshawb fawb thiab kev txhim kho. Peb cov khoom siv hluav taws xob siv hluav taws xob zoo tshaj plaws tau khav theeb cov duab zoo tshaj plaws ntawm txhua lub xov tooj ntawm tes muaj nyob hauv khw. Thaum ua ke nrog lub xeev - ntawm - lub - kos duab software pob thiab kuaj automation kev daws teeb meem, lawv muab kev txhawb nqa zoo tshaj plaws hauv koj daim ntawv thov tshwj xeeb.

Hauv txoj kev loj hlob ntawm cov kab ntawm tes, muaj ntau yam kev siv xws li ib leeg - cell cloning, kev txheeb xyuas ntawm monoclonality, taug qab ntawm CRISPR / Cas9, kev soj ntsuam ntawm kev hloov pauv kev ua haujlwm, kev saib xyuas ntawm cell viability, ntsuas PAIA protein titer thiab glycosylation, thiab fluorescent - qhib. Ib leeg-cell cloning (FASCC).

Rau kev tshawb nrhiav mob qog noj ntshav thiab kev tshawb nrhiav tshuaj, peb muaj cov haujlwm xws li 3D spheroid imaging, toxicity testing, IC50 study, tracking of cell expansion, monitoring of apoptosis, characterization of the nucleus, conducting wound - healing and migration assys, analysis of H2AX - DNA puas, thiab soj ntsuam ntawm lub voj voog ntawm tes thiab mitosis.

Hauv kev tshawb fawb qia cell, peb ua haujlwm suav nrog suav iPS cov cheeb tsam, ua cov kev tshawb fawb fluorescent pluripotency, validating proliferation thiab cell migration, txheeb xyuas cell txawv, siv recombinant lectin probes, suav cornea hlwb, kuaj siRNA, thiab characterizing iPS - cell markers.

Hauv kev tiv thaiv kab mob, peb ua cov kev tshawb fawb ntawm B - hlwb thiab T - hlwb, ua cytotoxic T - lymphocyte kuaj, ntsuas tus pab T - hlwb thiab lawv cov subsets, thiab kawm cell tuag.

Hauv kev tshawb fawb tshuaj tiv thaiv, peb ua haujlwm xws li siv qhov kev tsom xam - tsim kev soj ntsuam (FFA) rau tus kab mob titer kom muaj nuj nqis, immunofluorescence foci assay (IFA) rau kev ntsuam xyuas kab mob kis kab mob, kev ntsuam xyuas kab mob plaque, tshawb xyuas kab mob pathogenesis los ntawm quantifying morphological hloov, txiav txim siab. kev ua haujlwm nrog fluorescence - txuas cov noob qhia, thiab quantifying cov nyhuv cytopathic (viral CPE).

Nyob - cell imaging plays lub luag hauj lwm tseem ceeb raws li ib tug analytical tool nyob rau hauv lub chaw soj nstuam mob siab rau biomedical kev tshawb fawb teb xws li cell biology, neurobiology, pharmacology, thiab kev loj hlob biology. Thaum kuaj xyuas cov hlwb thiab cov ntaub so ntswg, qhov twg photobleaching yog ib qho kev txhawj xeeb tseem ceeb, kev siv lub teeb pom kev zoo thiab lub sij hawm ntev ntev yog qhov tsim nyog. Txawm li cas los xij, cov xwm txheej no yuav tsum tau zam thaum lub sij hawm kuaj xyuas cov cell nyob. Hauv kev ua neej nyob - cell microscopy, qhov sib npaug feem ntau yuav tsum raug ntaus ntawm kev tau txais cov duab zoo thiab ua kom lub hlwb noj qab haus huv. Yog li ntawd, txhawm rau tiv thaiv kev siv lub teeb pom kev siab thiab lub sijhawm ntev, qhov kev daws teeb meem ntawm qhov chaw thiab lub cev ntawm lub cev hauv ib qho kev sim yog nquag txwv. Live - cell imaging for optical microscopy encompasses a broad range of contrast - enhanced imaging txoj kev. Feem ntau cov haujlwm tshawb fawb siv ib qho ntawm ntau hom fluorescence microscopy, thiab qhov no feem ntau ua ke nrog cov txheej txheem teeb pom kev zoo, uas yuav piav qhia tom qab. Kev vam meej tas mus li hauv cov tswv yim kev yees duab thiab kev tsim cov fluorescent probes txhim khu kev ua tau zoo ntawm txoj hauv kev no, kom ntseeg tau tias nyob - cell imaging tseem yog cov txheej txheem tseem ceeb hauv biology.

Ib qho kev txiav txim siab tseem ceeb yog kom paub tseeb tias cov hlwb nyob hauv lub xeev kom zoo thiab ua haujlwm ib txwm muaj thaum nyob rau theem ntawm lub tshuab ntsuas hluav taws xob nyob rau hauv lub teeb pom kev, tshwj xeeb tshaj yog nyob rau hauv muaj cov hluavtaws fluorophores lossis fluorescent proteins. Cov xwm txheej uas cov hlwb tau khaws cia rau ntawm lub tshuab tsom mus rau theem, txawm tias muaj qhov sib txawv, feem ntau txiav txim siab qhov ua tiav lossis tsis ua tiav ntawm kev sim.

Muaj ntau hom kab lis kev cai xov xwm ntawm tes muaj, uas yog tsim los ntawm cov txheej txheem biochemical tshwj xeeb ntawm cov hlwb. Cov xov xwm no muaj ntau yam xws li amino acids, vitamins, inorganic ntsev (minerals), kab kawm, nucleic acid Cheebtsam (bases thiab nucleosides), qab zib, tricarboxylic acid voj voog intermediates, lipids, thiab co-enzymes. Hauv cov ntaub so ntswg kab lis kev cai, ib kauj ruam tseem ceeb yog los tswj cov pa oxygen concentration, pH, buffering peev xwm, osmolarity, viscosity, thiab nto nro. Kev lag luam uas muaj cov qauv tsim tawm feem ntau muaj qhov taw qhia zas xim (piv txwv li, phenol liab) txhawm rau ua kom pom kev kwv yees ntawm kwv yees tus nqi pH. Yuav luag txhua kab ntawm tes yuav tsum muaj cov pa roj carbon dioxide thiab bicarbonate tsis siv los tswj cov pH. Cov hlwb yuav tsum tau coj mus rau hauv ib puag ncig hauv lub incubator uas muaj cov pa roj carbon dioxide me me (feem ntau yog 5 - 7%) los tswj cov concentration ntawm cov pa yaj. Rau kev ua neej nyob - xovtooj ntawm tes, muab qhov chaw tsim nyog nrog tus nqi ntawm cov pa roj carbon dioxide tuaj yeem nyuaj, thiab qhov no feem ntau xav tau kev siv cov kab lis kev cai tshwj xeeb uas tuaj yeem tswj cov huab cua. Cov pa oxygen xav tau sib txawv ntawm cov kab sib txawv ntawm tes, tab sis cov qib siab ntawm cov pa oxygen tensions feem ntau haum rau ntau haiv neeg. Hais txog osmolarity, feem ntau cov kab ntawm tes muaj qhov ua siab ntev rau osmotic siab thiab tuaj yeem loj hlob zoo nyob rau hauv osmolarity ntau ntawm 260 - 320 milliosmolar. Thaum cov hlwb loj hlob nyob rau hauv qhib - phaj kab lis kev cai los yog Petri tais diav, ib tug hypotonic nruab nrab yuav siv tau los daws qhov teeb meem ntawm evaporation.

Pab neeg ua haujlwm

Peb tshwj xeeb hauv kev siv cov cuab yeej kho qhov muag kho qhov muag rau thaj tsam ntawm cell biology. Rau kev tshawb fawb ntawm tes, kev soj ntsuam thiab lwm daim ntawv thov teb.Peb muaj qhov ua tiav kho qhov muag sim kev sim platform thiab ib pab pawg ntawm cov tub ntxhais hluas cov pob txha zoo.

Cov cuab yeej siv siab heev

Raws li kev sib koom ua ke ntawm cov khoom siv hauv kev lag luam thiab kev lag luam hauv Is Taws Nem, lub tuam txhab tau cog lus los tsim ib tiam tshiab ntawm cov khoom siv ntse ntse hauv chav kuaj.

Kev tshawb fawb ywj pheej thiab kev txhim kho

Raws li kev tsim kho tshiab ntawm pab pawg kev tshawb fawb thiab kev txhim kho muaj zog, EAST cov khoom lag luam tau txais kev ywj pheej tshawb fawb thiab kev tsim kho, kev tsim khoom ywj pheej, kev ywj pheej patents, thiab tau dhau ntau daim ntawv pov thawj xws li software monographs thiab cov qauv siv hluav taws xob.

Software zoo

Software tuning yog ua raws li kev siv tus cwj pwm ntawm cov neeg siv tshawb fawb tshawb fawb, thiab cov txiaj ntsig tau raug xa tawm raws li qhov xav tau ntawm cov ntawv tshawb fawb tshawb fawb thiab cov ntawv ceeb toom. Daim ntawv saib ua ntej cov ntaub ntawv tuaj yeem muab rov qab tau txhua lub sijhawm, thiab txhawb kev hloov pauv ntawm cov txiaj ntsig panoramic, uas yooj yim rau kev tshawb fawb thoob ntiaj teb.

Cim npe nrov: Suav teb ntse ntse scanning system, Tuam Tshoj nyob cell ntse scanning system manufacturers, lwm tus neeg, Hoobkas